Hello, I have gone through the following manuscripts
1. Kromdijk, J., Głowacka, K., Leonelli, L., Gabilly, S. T., Iwai, M., Niyogi, K. K., and Long, S. P. (2016). Improving photosynthesis and crop productivity by accelerating recovery from photoprotection. Science 354, 857-861.enter link description here
2. Hubbart, S., Smillie, I. R. A., Heatley, M., Swarup, R., Foo, C. C., Zhao, L., and Murchie, E. H. (2018). Enhanced thylakoid photoprotection can increase yield and canopy radiation use efficiency in rice. Communications Biology 1, 22.
I understand that NPQ flexibility allows the plant to buffer the light-induced changes in ETR and thus regulate photosynthetic metabolism. NPQ or PhiNPQ or NPQ(T) or qE can be used as potential selection criteria for improving crop productivity. My Question is Using MultispeQ, which protocol is best for phenotyping wheat lines.
I would recommend using the Leaf Photosynthesis RIDES protocol instead of the Leaf Photosynthesis, since the ECS data, that is collected using RIDES, has been improved.